LOCUS       sequence-53010-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RPS6KA1
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggttttt
      121 agtgacggct acgtggtaaa ggagacaatt ggtgtgggct cctactctga gtgcaagcgc
      181 tgtgtccaca aggccaccaa catggagtat gctgtcaagg tcattgataa gagcaagcgg
      241 gatccttcag aagagattga gattcttctg cggtatggcc agcaccccaa catcatcact
      301 ctgaaagatg tgtatgatga tggcaaacac gtgtacctgg tgacagagct gatgcggggt
      361 ggggagctgc tggacaagat cctgcggcag aagttcttct cagagcggga ggccagcttt
      421 gtcctgcaca ccattggcaa aactgtggag tatctgcact cacagggggt tgtgcacagg
      481 gacctgaagc ccagcaacat cctgtatgtg gacgagtccg ggaatcccga gtgcctgcgc
      541 atctgtgact ttggttttgc caaacagctg cgggctgaga atgggctcct catgacacct
      601 tgctacacag ccaactttgt ggcgcctgag gtgctgaagc gccagggcta cgatgaaggc
      661 tgcgacatct ggagcctggg cattctgctg tacaccatgc tggcaggata tactccattt
      721 gccaacggtc ccagtgacac accagaggaa atcctaaccc ggatcggcag tgggaagttt
      781 accctcagtg ggggaaattg gaacacagtt tcagagacag ccaaggacct ggtgtccaag
      841 atgctacacg tggatcccca ccagcgcctc acagctaagc aggttctgca gcatccatgg
      901 gtcacccaga aagacaagct tccccaaagc cagctgtccc accaggacct acagcttgtg
      961 aagggagcca tggctgccac gtactccgca ctcaacagct ccaagcccac ccc
//
