LOCUS       sequence-53023-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: UGDH
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..150
                     /codon_start=1
                     /product="solubility-enhancement tag derived from T7 phage 
                     gene 10B"
                     /label=SET1
                     /translation="DPEEASVTSTEETLTPAQEAARTRAANKARKEAELAAATAEQ"
     CDS             157..267
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             298..318
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttaagaag gagatataca tatggacccc gaagaggcga gtgttacttc aacagaagaa
       61 accttaacgc cagcacagga ggccgcacgc acccgcgctg ctaacaaagc ccgaaaggaa
      121 gctgagttgg ctgctgccac cgctgaacaa actagtgacg agaagaccac cggctggcgg
      181 ggcggccacg tggtggaggg cctggccggc gagctggagc agctgcgggc caggctggag
      241 caccaccctc agggccagcg ggagccctcc ggcggctgca agctgggcct gggtaccgag
      301 aacctgtact tccaatccat gtttgaaatt aagaagatct gttgcattgg tgcaggctat
      361 gttggaggac ccacatgtag tgtcattgct catatgtgtc ctgaaatcag ggtaacggtt
      421 gttgatgtca atgaatcaag aatcaatgcg tggaattctc ctacacttcc tatttatgag
      481 ccaggactaa aagaagtggt agaatcctgt cgaggaaaaa atcttttttt ttctaccaat
      541 attgatgatg ccatcaaaga agctgatctt gtatttattt ctgtgaatac tccaacaaaa
      601 acctatggaa tggggaaagg ccgggcagca gatctgaagt atattgaagc ttgtgctaga
      661 cgcattgtgc aaaactcaaa tgggtacaaa attgtgactg agaaaagcgc agttcc
//
