LOCUS       sequence-53024-e         400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 400)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/Parkin
FEATURES             Location/Qualifiers
     source          1..400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(52..143)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(110..127)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        complement(249..358)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 gtggtggtgg tggtggtgct cgagtgcggc cgcaagctta gaggatccga tatttatagg
       61 tttttttatt acaaaactgt tacgaaaaca gtaaaatact tatttatttg cgagatggtt
      121 atcattttaa ttatctccat gatcagatcc gggggatcga tcctctaggt tttattacta
      181 gacacaaaaa accaacacac acatctaatg aaaataaaca tcttttatta ctagtcccgg
      241 ggatctgtga ttgtaaataa aatgtaattt acagtatagt attttaatta atatacaaat
      301 gatttgataa taattcttat ttaactataa tatattgtgt tgggttgaat taaaggtcgg
      361 gccctttatt ctaggtttat tggatcatgg agataattaa
//
