LOCUS       sequence-53029-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus_Sumostar/LRRK2 1867-2186_G2019S
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..402
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             409..432
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             433..453
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taaccatctc gcaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
       61 cctataaata taggatctat gggtcatcac catcatcatc acgggtccct gcaggactca
      121 gaagtcaatc aagaagctaa gccagaggtc aagccagaag tcaagcctga gactcacatc
      181 aatttaaagg tgtccgatgg atcttcagag atcttcttca agatcaaaaa gaccactcct
      241 ttaagaaggc tgatggaagc gttcgctaaa agacagggta aggaaatgga ctccttaacg
      301 ttcttgtacg acggtattga aattcaagct gatcagaccc ctgaagattt ggacatggag
      361 gataacgata ttattgaggc tcacagagaa cagattggag gatccatgca ccaccatcac
      421 caccatcacc acgagaacct ttatttccaa ggaaacatca tgctcaacaa cgatgagctc
      481 gaatttgaac aagctccaga attcctgctc ggcgacggct ctttcggctc cgtataccgt
      541 gctgcttatg aaggcgaaga agtcgccgta aagatcttca acaagcacac ctcgctccgt
      601 ctgctccgtc aggaactggt cgttctgtgt catctgcacc atccgagcct catctccctg
      661 ctggccgctg gcatccgtcc tcgtatgctg gtgatggagc tggcatccaa gggctctctc
      721 gaccgcctgc tgcagcagga caaggcttct ctgacccgta ctctgcagca tcgtatcgct
      781 ctgcatgtag ctgacggcct gcgttatctt cattctgcaa tgatcattta tcgcgacctg
      841 aaacctcata
//
