LOCUS       sequence-53030-e        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             522..539
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(592..610)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      606..653
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      690..1048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(777..796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     987..1008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgaccgtt
      121 gatccgattg ccgtgtgtca tgaactgtat aataccattc gcgattataa agatgaacag
      181 ggtcgcctgc tgtgcgaact gttcattcgc gccccgaaac gtcgcaatca gccggattat
      241 tatgaagtgg ttagccagcc gatcgatctg atgaaaattc agcagaaact gaaaatggaa
      301 gaatatgatg atgtgaacct gctgaccgcg gattttcagc tgctgtttaa taatgcgaaa
      361 agctattata aaccggatag cccggaatat aaagccgcgt gcaaactgtg ggatctgtat
      421 ctgcgtaccc gcaatgaatt tgtgcagaaa ggtgaatgac agtaaaggtg gatacggatc
      481 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      541 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      601 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      661 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      841 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      901 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     1021 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttc
//
