LOCUS       sequence-53032-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00577
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(321..420)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(437..456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(438..456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(461..477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(461..477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(579..598)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(657..676)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
       61 gccaactttg tacaaaaaag ttggcaccat gaactcgggc atcagctcgg gcagccatgt
      121 taaggatacc gcatactacg agaggcgtcg caagaacaat gctgccgcca agaagtcgcg
      181 cgatcgccgc cgcatcaagg aggatgagat cgccatcagg gccgcctatc tggaacgcca
      241 gaacatcgag ctcttgtgcc ggatcgacgc cctcgaggtc cagctggccg ccatcacctc
      301 cgccaaagtt accaccgcct tgccaacttt cttgtacaaa gttggcatta taagaaagca
      361 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      421 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
      481 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      541 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      601 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
      661 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgg
//
