LOCUS       sequence-53039-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus_Sumostar/LRRK2 1867-2141
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             111..398
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             405..428
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             429..449
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catctcgcaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
       61 taaatatagg atctatgggt catcaccatc atcatcacgg gtccctgcag gactcagaag
      121 tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt
      181 taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa
      241 gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaacgttct
      301 tgtacgacgg tattgaaatt caagctgatc agacccctga agatttggac atggaggata
      361 acgatattat tgaggctcac agagaacaga ttggaggatc catgcaccac catcaccacc
      421 atcaccacga gaacctttat ttccaaggaa acatcatgct caacaacgat gagctcgaat
      481 ttgaacaagc tccagaattc ctgctcggcg acggctcttt cggctccgta taccgtgctg
      541 cttatgaagg cgaagaagtc gccgtaaaga tcttcaacaa gcacacctcg ctccgtctgc
      601 tccgtcagga actggtcgtt ctgtgtcatc tgcaccatcc gagcctcatc tccctgctgg
      661 ccgctggcat ccgtcctcgt atgctggtga tggagctggc atccaagggc tctctcgacc
      721 gcctgctgca gcaggacaag gcttctctga cccgtactct gcagcatcgt atcgctctgc
      781 atgtagctga cggcctgcgt tatcttcatt ctgcaatgat catttatcgc gacctgaaac
      841 ctcataatgt actgctgttt actctgtacc cgaacgctgc catcatcgct aa
//
