LOCUS       sequence-53043-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus_Sumostar/LRRK2 1867-2176
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..388
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             395..418
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             419..439
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatatagg
       61 atctatgggt catcaccatc atcatcacgg gtccctgcag gactcagaag tcaatcaaga
      121 agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt taaaggtgtc
      181 cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa gaaggctgat
      241 ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaacgttct tgtacgacgg
      301 tattgaaatt caagctgatc agacccctga agatttggac atggaggata acgatattat
      361 tgaggctcac agagaacaga ttggaggatc catgcaccac catcaccacc atcaccacga
      421 gaacctttat ttccaaggaa acatcatgct caacaacgat gagctcgaat ttgaacaagc
      481 tccagaattc ctgctcggcg acggctcttt cggctccgta taccgtgctg cttatgaagg
      541 cgaagaagtc gccgtaaaga tcttcaacaa gcacacctcg ctccgtctgc tccgtcagga
      601 actggtcgtt ctgtgtcatc tgcaccatcc gagcctcatc tccctgctgg ccgctggcat
      661 ccgtcctcgt atgctggtga tggagctggc atccaagggc tctctcgacc gcctgctgca
      721 gcaggacaag gcttctctga cccgtactct gcagcatcgt atcgctctgc atgtagctga
      781 cggcctgcgt tatcttcatt ctgcaatgat catttatcgc gacctgaaac ctcataatgt
      841 actgctgttt actctgtacc cgaacgctgc
//
