LOCUS       sequence-53051-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus_Sumostar/LRRK2 1867-2161_T2031E
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..390
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             397..420
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             421..441
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatata
       61 ggatctatgg gtcatcacca tcatcatcac gggtccctgc aggactcaga agtcaatcaa
      121 gaagctaagc cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa tttaaaggtg
      181 tccgatggat cttcagagat cttcttcaag atcaaaaaga ccactccttt aagaaggctg
      241 atggaagcgt tcgctaaaag acagggtaag gaaatggact ccttaacgtt cttgtacgac
      301 ggtattgaaa ttcaagctga tcagacccct gaagatttgg acatggagga taacgatatt
      361 attgaggctc acagagaaca gattggagga tccatgcacc accatcacca ccatcaccac
      421 gagaaccttt atttccaagg aaacatcatg ctcaacaacg atgagctcga atttgaacaa
      481 gctccagaat tcctgctcgg cgacggctct ttcggctccg tataccgtgc tgcttatgaa
      541 ggcgaagaag tcgccgtaaa gatcttcaac aagcacacct cgctccgtct gctccgtcag
      601 gaactggtcg ttctgtgtca tctgcaccat ccgagcctca tctccctgct ggccgctggc
      661 atccgtcctc gtatgctggt gatggagctg gcatccaagg gctctctcga ccgcctgctg
      721 cagca
//
