LOCUS       sequence-53064-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD3
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             768..788
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             789..806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             807..830
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      967..1013
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccctctagaa tattttgttt accttaagaa ggagatatac tatgcgactg ctgacgtggg
       61 atgtgaagga cacgctgctc aggctccgcc accccttagg ggaggcctat gccaccaagg
      121 cccgggccca tgggctggag gtggagccct cagccctgga acaaggcttc aggcaggcat
      181 acagggctca gagccacagc ttccccaact acggcctgag ccacggccta acctcccgcc
      241 agtggtggct ggatgtggtc ctgcagacct tccacctggc gggtgtccag gatgctcagg
      301 ctgtagcccc catcgctgaa cagctttata aagacttcag ccacccctgc acctggcagg
      361 tgttggatgg ggctgaggac accctgaggg agtgccgcac acggggtctg agactggcag
      421 tgatctccaa ctttgaccga cggctagagg gcatcctggg gggccttggc ctgcgtgaac
      481 acttcgactt tgtgctgacc tccgaggctg ctggctggcc caagccggac ccccgcattt
      541 tccaggaggc cttgcggctt gctcatatgg aaccagtagt ggcagcccat gttggggata
      601 attacctctg cgattaccag gggcctcggg ctgtgggcat gcacagcttc ctggtggttg
      661 gcccacaggc actggacccc gtggtcaggg attctgtacc taaagaacac atcctcccct
      721 ctctggccca tctcctgcct gcccttgact gcctagaggg ctcagcagag aacctctact
      781 tccaatcgca ccatcatcac caccatgatt acaaggatga cgacgataag tgaggatccg
      841 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaaccacc accaccactg
      901 agatccggct gctaacaaag ccccgaaagg aagctgaaat tggctgctgc caccgctgaa
      961 caataactag cataacccct tggggcctct aacgggtctt gaggggtttt ttgctgaaag
     1021 ga
//
