LOCUS       sequence-53067-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pSTC0
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      25..96
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      112..139
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    146..166
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      167..224
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(248..265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(499..518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
       61 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      121 tcgggtgggc ctttctgcgt ttatatacta gtagcggccg ctgcagtccg gcaaaaaagg
      181 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      241 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      421 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      781 acaggattag cagagcgagg tatgtaggcg
//
