LOCUS       sequence-53068-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..64
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             454..471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(524..542)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      538..585
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(709..728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcaccatcat catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca
       61 atccatgcag ctgtatgata ccgtgcgtag ctgccgcaac aaccagggcc agctgattgc
      121 ggaaccgttc tatcatctgc cgagcaaaaa aaaatatccg gattattatc agcagattaa
      181 aatgccgatc agcctgcagc agattcgcac caaactgaaa aatcaggaat atgaaaccct
      241 ggatcacctg gaatgcgatc tgaacctgat gtttgaaaac gcgaaacgtt ataacgtgcc
      301 gaatagcgcg atctataaac gtgttctgaa actgcagcag gttatgcagg ccaaaaaaaa
      361 agaactggcg cgtcgcgatg atattgaatg acagtaaagg tggatacgga tccgaattcg
      421 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      481 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      541 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      601 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      661 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      721 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      781 tccctttagg gttccgattt agtgctttac ggcaccctcg acccccaaaa aacttggatt
      841 taggggtgga tggttcacgt agtgggccat cgccctgaat agacgggttt tttcgccctt
      901 tga
//
