LOCUS       sequence-53073-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAST3
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             462..479
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(532..550)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      546..593
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      630..987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(717..736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     926..947
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcgtcc gccgattgtg
      121 attcatagca gcggcaaaaa atatggcttt agcctgcgcg ccattcgcgt gtatatgggt
      181 gatagcgatg tttataccgt tcatcatgtg gtgtggagcg tggaagatgg cagcccggcc
      241 caggaagcgg gcctgcgtgc gggcgatctg attacccata ttaacggcga aagcgtgctg
      301 ggcctggttc acatggatgt ggtggaactg ctgctgaaaa gcggcaataa aattagcctg
      361 cgtaccaccg cgctggaaaa taccgaaacc agcgtttgac agtaaaggtg gatacggatc
      421 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      481 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      541 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      601 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaactt
      841 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      901 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      961 cctatctcgg tctattcttt tgatttat
//
