LOCUS       sequence-53077-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX3
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..82
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             521..538
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(591..609)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      605..652
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(776..795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag atctgggtac
       61 cgagaacctg tacttccaat ccatggcggc gggggcggct gaggcagctg tagcggccgt
      121 ggaggaggtc ggctcagccg ggcagtttga ggagctgctg cgcctcaaag ccaagtccct
      181 ccttgtggtc catttctggg caccatgggc tccacagtgt gcacagatga acgaagttat
      241 ggcagagtta gctaaagaac tccctcaagt ttcatttgtg aagttggaag ctgaaggtgt
      301 tcctgaagta tctgaaaaat atgaaattag ctctgttccc acttttctgt ttttcaagaa
      361 ttctcagaaa atcgaccgat tagatggtgc acatgcccca gagttgacca aaaaagttca
      421 gcgacatgca tctagtggct ccttcctacc cagcgctaaa gtaaaggtgg atacggatcc
      481 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      541 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      601 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      661 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaaa
      841 tcgggggctc ccctttaggg ttccgattta gtgcttttac cggcacctcg aacccc
//
