LOCUS       sequence-53080-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pSTC1
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(96..773)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             780..791
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aaggcccacc cgtgaaggtg agccagtgag ttgattgcta cgtaattagt tagttagccc
       61 ttagtgactc gaattcgcgg ccgcttctag agttattaag caccggtgga gtgacgacct
      121 tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgggaggt gatgtccagt
      181 ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt ttttagccat gtaggtggtt
      241 ttaacttcag cgtcgtagtg accaccgtct ttcagtttca gacgcatttt gatttcacct
      301 ttcagagcac cgtcttccgg gtacatacgt tcggtggaag cttcccaacc catggttttt
      361 ttctgcataa ccggaccgtc ggacgggaag ttggtaccac gcagtttaac tttgtagatg
      421 aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa caacaccacc gtcttcgaag
      481 ttcataacac gttcccattt gaaaccttcc gggaaggaca gtttcaggta gtccgggatg
      541 tcagccgggt gtttaacgta agctttggaa ccgtactgga actgcgggga caggatgtcc
      601 caagcgaacg gcagcggacc acctttggta actttcagtt tagcggtctg ggtaccttcg
      661 tacggacgac cttcaccttc accttcgatt tcgaactcgt gaccgttaac ggaaccttcc
      721 atacgaactt tgaaacgcat gaactctttg ataacgtctt cggaggaagc catctagtat
      781 ttctcctctt tctctagtg
//
