LOCUS       sequence-53081-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GMFG
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             582..599
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(652..670)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      666..713
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(837..856)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggtgtgc
      121 gaggtagacc cagagctaac agaaaagctg aggaaattcc gcttccgaaa agagacagac
      181 aatgcagcca tcataatgaa ggtggacaaa gaccggcaga tggtggtgct ggaggaagaa
      241 tttcagaaca tttccccaga ggagctcaaa atggagttgc cggagagaca gcccaggttc
      301 gtggtttaca gctacaagta cgtgcatgac gatggccgag tgtcctaccc tttgtgtttc
      361 atcttctcca gccctgtggg ctgcaagccg gaacaacaga tgatgtatgc agggagtaaa
      421 aacaggctgg tgcagacagc agagctcaca aaggtgttcg aaatccgcac cactgatgac
      481 ctcactgagg cctggctcca agaaaagttg tctttctgac agtaaaggtg gatacggatc
      541 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      601 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      661 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      721 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      781 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      841 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      901 tcgggggctc cctttagg
//
