LOCUS       sequence-53088-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MACROD1
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..390
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             397..417
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      494..503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtatgagcg ataaaattat tcacctgact gacgacagtt ttgacacgga tgtactcaaa
      121 gcggacgggg cgatcctcgt cgatttctgg gcagagtggt gcggtccgtg caaaatgatc
      181 gccccgattc tggatgaaat cgctgacgaa tatcagggca aactgaccgt tgcaaaactg
      241 aacatcgatc aaaaccctgg cactgcgccg aaatatggca tccgtggtat cccgactctg
      301 ctgctgttca aaaacggtga agtggcggca accaaagtgg gcgcactgtc taaaggtcag
      361 ttgaaagagt tcctcgacgc taacctggcc ggtaccgaga acttgtactt ccaatccatg
      421 tcggcgggag ttggggcgtg gggggcggcg gcggtggggc ggacagccgg ggtgcgcact
      481 tgggcccccc tggccatggc ggcgaaggtg gacctgagca cctccaccga ctggaaggag
      541 gcgaaatcct ttctgaaggg cctgagtgac aagcagcggg aggaacatta cttctgcaag
      601 gactttgtca ggctgaagaa gatcccgaca tggaaggaga tggcgaaagg ggtggctgtg
      661 aaggtggagg agcccaggta taaaaaggac aagcagctca atgagaaaat ctccctgctc
      721 cgcagcgaca tcaccaagct ggaggtggac gccatcgtca acgccgccaa cagctccctg
      781 ctcggaggcg gtggcgtgga cggctgcatt catcgggccg ccggccccct gcttaccgac
      841 gagtgccgga ccctgcagag ctgtaagact ggcaaggcca agatcaccgg cggctatcgg
      901 ctcccggcca agtacgtcat ccacacagtg gggccc
//
