LOCUS       sequence-53089-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GAK
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tggcgggtcc aggctccctg
      121 ggcggtgctt ccggccgcga ccagagtgac ttcgtggggc agacggtgga actgggcgag
      181 ctgcggctgc gggtgcggcg ggtcctggcc gaaggagggt ttgcatttgt gtatgaagct
      241 caagatgtgg ggagtggcag agagtatgca ttaaagaggc tattatccaa tgaagaggaa
      301 aagaacagag ccatcattca agaagtttgc ttcatgaaaa agctttccgg ccacccgaac
      361 attgtccagt tttgttctgc agcgtctata ggaaaagagg agtcagacac ggggcaggct
      421 gagttcctct tgctcacaga gctctgtaaa gggcagctgg tggaattttt gaagaaaatg
      481 gaatctcgag gccccctttc gtgcgacacg gttctgaaga tcttctacca gacgtgccgc
      541 gccgtgcagc acatgcaccg gcagaagccg cccatcatcc acagggacct caaggttgag
      601 aacttgttgc ttagtaacca agggaccatt aagctgtgtg actttggcag tgccacgacc
      661 atctcgcact accctgacta cagctggagc gcccagaggc gagccctggt ggaggaagag
      721 atcacgagga atacaacacc aatgtataga acaccagaaa tcatagactt gtattccaac
      781 ttcccgatcg gcgagaagca ggatatctgg gccctgggct gcatcttgta cctgctgtgc
      841 ttccggcagc acccttttga ggatggagcg aaa
//
