LOCUS       sequence-53112-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00608
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(531..630)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(646..663)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(646..662)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..677)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaaa gtagcactgg gatttgcact
      121 tgccgccgct gaagtagagg cctccaggga tgtacctgct ccgctcgatg tctgtgtcgc
      181 tcgagaggct ttgtaggcca gctctagggt ctggttaatc gtatgcatgt ccccctctat
      241 gctgcgtgcc cagttctcca cgtcgccgag atcctttagg gcagtgctga attggtcgat
      301 gagctgcagc cattggtggg tctgcttggc gaaattggtg gcgcccaagt gcagttgctt
      361 tgcctctgcg tccagccgct tctggttcag gtaggcctga gccacgccca catttagggt
      421 gtccaccaga gactgagtga gctcattgga cgcttctatt gcctcctttc gacgcacctc
      481 ctgttcctgt tttcgcttcg cctgctcctt gtgatgctcc ttaaccatgg tgccaacttt
      541 tttgtacaaa gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt
      601 cactatcagt caaaataaaa tcattatttg gggcccgagc ttaagactgg ccgtcgtttt
      661 acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca
      721 ggcatcaaat aaaacgaaag gctcagtcg
//
