LOCUS       sequence-53113-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00608
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(519..618)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(635..654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(636..654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(659..675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(659..675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
       61 gccaactttg tacaaaaaag ttggcaccat ggttaaggag catcacaagg agcaggcgaa
      121 gcgaaaacag gaacaggagg tgcgtcgaaa ggaggcaata gaagcgtcca atgagctcac
      181 tcagtctctg gtggacaccc taaatgtggg cgtggctcag gcctacctga accagaagcg
      241 gctggacgca gaggcaaagc aactgcactt gggcgccacc aatttcgcca agcagaccca
      301 ccaatggctg cagctcatcg accaattcag cactgcccta aaggatctcg gcgacgtgga
      361 gaactgggca cgcagcatag agggggacat gcatacgatt aaccagaccc tagagctggc
      421 ctacaaagcc tctcgagcga cacagacatc gagcggagca ggtacatccc tggaggcctc
      481 tacttcagcg gcggcaagtg caaatcccag tgctactttg ccaactttct tgtacaaagt
      541 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
      601 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
      661 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
      721 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaataca
//
