LOCUS       sequence-53114-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V43 pHIPPY GFP22
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..132)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     rep_origin      192..780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     681..700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcttgtgg ctagcgtctc aaaaagagct gtttctgagg agcctttttg agacggtctc
       61 aggatccact tataagattc ccaaatccaa agacatttca cgtttatggt gatttcccag
      121 aacacatagc gacatgcaaa tgcggccgcc cactgagcgt cagaccccgt ggaaaagatc
      181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      781 aacg
//
