LOCUS       sequence-53118-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL13
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(24..29,30..47,48..54)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     67..89
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     86..102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     86..102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             98..505
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     121..141
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        123..141
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(188..207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(189..207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(217..234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(217..233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(226..248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
       61 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      121 gcaattaacc ctcactaaag ggaacaaaag ctggaggacc tcgagggggg gcccggtacc
      181 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
      241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      361 gaatggcgaa tgggacgcgc cctgtagcgg cgcccgtctc caggggggca cccgtcagtg
      421 cattgcgcga tgcatgcact gcctccattg cggtcacgcc gccacgtttt gcaatcttca
      481 caagttggcc tgtgtccaac tgtaacggcg gacctctcaa ctcccccgca tccgtgagca
      541 aggcctccag ggcgcgtgcg ccggaccact gtttgccgac gccaacgatg tcttcgtgtg
      601 tcgcctctgg caacgccgtg attatgtgct gatacgtgac agcgacggtc cctaacgctg
      661 ccgggtgttg gctgagcgca acgatgtgcg cgtgtgtaaa cccatggccc accagtgcct
      721 cgtggtgctg cgccactgtc gaacgcacct tcggtttgat cttctcttgc tgctgctgac
      781 tgtagccgag cgtgcgtaga tccacctgcg cggccggcga a
//
