LOCUS       sequence-53123-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL13
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             232..252
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             253..279
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagactc gagcggccgc
      121 cactgtgctg gatatctgca gaattccacc acactggact agtggatcta actcgagaaa
      181 gatattgtat atatcgtaac aataggaggt tcaacaatgg cttcctcccc tccaaagaaa
      241 aagagaaagg ttagttggaa ggacgcaagt ggttggtcta gaatgcatgc ggatcccatt
      301 cgtccgcgca ggccaagtcc tgcccgcgag cttctgcccg gaccccaacc ggatagggtt
      361 cagccgactg cagatcgtgg ggtgtctgcg cctgctggca gccctctgga tggcttgccc
      421 gctcggcgga cggtgtcccg gacccggctg ccatctcccc ctgcgccctc acctgcgttc
      481 tcggcgggca gcttcagcga tctgctccgt ccgttcgatc cgtcgcttct tgatacatcg
      541 cttcttgatt cgatgcctgc cgtcggcacg ccgcatacag cggctgcccc agcagagtgg
      601 gatgaggcgc aatcggctct gcgtgcagcc gatgacccgc cacccaccgt gcgtgtcgct
      661 gtcactgccg cgcggccgcc gcgcgccaag ccggccccgc gacggcgtgc tgcgcaaccc
      721 tccgacgctt cgccggccgc gcag
//
