LOCUS       sequence-53124-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M60 pPET28a-Xenopus beta catenin domains NR7
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             87..119
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             174..209
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ttgtttactt tagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
       61 gcggcctggt gccgcgcggc agccatatgg ctagcatgac tggtggacag caaatgggtc
      121 gcggatccat ggcaactcaa gcagatctaa tggagctgga catggccatg gagccagacc
      181 gaaaagcagc agtcagtcac tggcagcaac agtcttacct ggatgctggg attcatgctg
      241 gagcaaccgc cacagcacca gctttgagtg gcaaaggaaa cccagaggat gaagatgtgg
      301 ataccaacca agttttgtat gagtgggagc agggcttctc tcagtccttc actcaagatc
      361 aagtggctga tattgatggc cagtacgcca tgacaagagc ccagcgagtt cgtgctgcaa
      421 tgtttccaga aacccttgat gaaggcatgc agattccatc cacacaattt gactctgcac
      481 acccaacaaa tgtgcaacgt ttagcagagc cttcccagat gctcaaacac gctgtggtca
      541 acttgatcaa ttaccaggat gatgctgaat tggccactcg agcaatcccc gagctgacga
      601 aactgcttaa tgacgaggac caggttgtag ttaacaaggc tgctgtaatg gttcaccagc
      661 tgtcaaagaa ggaagcctca cgccatgcta taatgcgctc accacagatg gtgtctgcta
      721 tagttcgtac aatgcagaac acaaatgatg tagagactgc acgatgcact gcgggaacac
      781 ttcacaacct ttctcaccat cgtgaaaggc
//
