LOCUS       sequence-53133-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_F1908A_F2079G
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(938..958)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtggtggtgg tggtggtgct cgagtgctta ctaaagctta tcatcagtta tggtgagtag
       61 caaccataca ttccacgata acattcttag gcaggagaat acggcgagtc agacagacga
      121 gttcagcgct gttaaggatg tcgaacactt gagcagaggt tggacgttcc tgagggtttt
      181 ctttaaggca ctgtttgatg agcttctcta ccatcggcca cggagcgcaa ccgtattctt
      241 taaccgggtc tggcagcttg ccctggattt ccagttcgtc gaattcgtta gggcccttca
      301 gaccttctac gatacggccg ccggtagtca ggatatcgta caggagcaga ccgaacgagt
      361 aaacgtcagc ttgctggttg taaataacgt tgccacgagc cacttctgga gcacggaaac
      421 ccggcgtacc ttcgcttgtt ttaatgccca tgcggcagca gtactgcgca ataccatagt
      481 ctgcaatctt agcgatgatg gcagcgttcg ggtacagagt aaacagcagt acattatgag
      541 gtttcaggtc gcgataaatg atcattgcag aatgaagata acgcaggccg tcagctacat
      601 gcagagcgat acgatgctgc agagtacggg tcagagaagc cttgtcctgc tgcagcaggc
      661 ggtcgagaga gcccttggat gccagctcca tcaccagcat acgaggacgg atgccagcgg
      721 ccagcaggga gatgaggctc ggatggtgca gatgacacag aacgaccagt tcctgacgga
      781 gcagacggag cgaggtgtgc ttgttagcga tctttacggc gacttcttcg ccttcataag
      841 cagcacggta tacggagccg aaagagccgt cgccgagcag gaattctgga gcttgttcaa
      901 attcgagctc atcgttgttg agcatgatgt tggatccgcc ctggaaatac aggttctcct
      961 ccagcacagc ggcagctgac ccgg
//
