LOCUS       sequence-53153-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_F1908A_L1936R_V2048S
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..92
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             117..767
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPP"
ORIGIN
        1 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatatag
       61 gatctatgca ccaccatcac caccatcacc acgagaacct ttatttccaa ggatcaatgg
      121 caccgatcct ggggtattgg aagatcaagg gcctagtgca gcccactcgt ctgctcctgg
      181 agtacctaga agagaagtat gaggagcacc tttatgagcg ggacgagggt gacaagtggc
      241 gcaataagaa attcgagcta ggcttggaat tccccaatct gccttattac atcgatggcg
      301 acgttaagtt gactcagtcc atggccatca tccgctacat cgccgacaag cacaatatgc
      361 taggcgggtg cccgaaggag cgagctgaga tctctatgtt ggaaggggcc gtgctggata
      421 tccgctacgg cgtgagccgc attgcatact caaaggattt cgagaccctc aaggttgact
      481 tcctgtcaaa gctgcctgaa atgctaaaga tgttcgaaga ccgtctctgt cataagacct
      541 acctgaacgg ggaccacgtg acacacccgg actttatgtt gtatgacgct ctggacgtcg
      601 tgctttatat ggaccctatg tgcctggacg cttttcccaa actagtgtgt ttcaagaaac
      661 gtatcgaagc tatccctcag attgataagt acctgaagtc ctcaaagtac atcgcctggc
      721 cccttcaagg ttggcaagcc acatttggcg gtggcgacca tcctcccaa
//
