LOCUS       sequence-53160-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M61 pPET28c-Xenopus beta catenin domains R10C
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcggatcc tttgtccagc caatcatgct ccactgcgtg aacagggtgc cattccccga
      181 cttgttcagt tgcttgttcg tgcacaccag gacacacaga ggcgcacttc aatgggtgga
      241 acacaacagc agtttgtgga gggtgtccgc atggaggaga ttgttgaagg ctgcacaggt
      301 gctcttcaca ttttggctcg tgacatccac aaccgaattg taatcagagg tcttaacacc
      361 attccattat ttgtgcagtt gctgtactct cctattgaaa atatccaaag agtggcagca
      421 ggcgtgcttt gtgagctggc acaagacaaa gaggcagctg aggctattga agctgaaggt
      481 gcaactgctc ctcttactga gctgcttcac tctagaaatg aaggtgtcgc aacttatgca
      541 gctgctgttc tgttccgtat gtctgaggac aaaccccagg actacaagaa gcgtctgtca
      601 gttgagttga ccagctctct cttcaggacc gagccaatgc catggaatga ggctgcagac
      661 ctgggtcttg atattggtgc ccaaggcgaa cctcttggct acagacagga tgattcaagc
      721 taccgatctt tccatgctgc tggctatggt caggatgcaa tgggtatgga ctccatgatg
      781 gatcatgaca tgggaggtca ccacccaggg
//
