LOCUS       sequence-53163-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_F1908A_L1936R_F2079G_I2131Q
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..102
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             127..780
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
ORIGIN
        1 ccatctcgca ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
       61 ataaatatag gatctatgca ccaccatcac caccatcacc acgagaacct ttatttccaa
      121 ggatcaatgg caccgatcct ggggtattgg aagatcaagg gcctagtgca gcccactcgt
      181 ctgctcctgg agtacctaga agagaagtat gaggagcacc tttatgagcg ggacgagggt
      241 gacaagtggc gcaataagaa attcgagcta ggcttggaat tccccaatct gccttattac
      301 atcgatggcg acgttaagtt gactcagtcc atggccatca tccgctacat cgccgacaag
      361 cacaatatgc taggcgggtg cccgaaggag cgagctgaga tctctatgtt ggaaggggcc
      421 gtgctggata tccgctacgg cgtgagccgc attgcatact caaaggattt cgagaccctc
      481 aaggttgact tcctgtcaaa gctgcctgaa atgctaaaga tgttcgaaga ccgtctctgt
      541 cataagacct acctgaacgg ggaccacgtg acacacccgg actttatgtt gtatgacgct
      601 ctggacgtcg tgctttatat ggaccctatg tgcctggacg cttttcccaa actagtgtgt
      661 ttcaagaaac gtatcgaagc tatccctcag attgataagt acctgaagtc ctcaaagtac
      721 atcgcctggc cccttcaagg ttggcaagcc acatttggcg gtggcgacca tcctcccaag
      781 tcaga
//
