LOCUS       sequence-53165-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_F1908A_L1936R_V2048S_F2079G_I2...
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..72
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttcgtaaca gttttgtaat aaaaaaacct ataaatatag gatctatgca ccaccatcac
       61 caccatcacc acgagaacct ttatttccaa ggatcaatgg caccgatcct ggggtattgg
      121 aagatcaagg gcctagtgca gcccactcgt ctgctcctgg agtacctaga agagaagtat
      181 gaggagcacc tttatgagcg ggacgagggt gacaagtggc gcaataagaa attcgagcta
      241 ggcttggaat tccccaatct gccttattac atcgatggcg acgttaagtt gactcagtcc
      301 atggccatca tccgctacat cgccgacaag cacaatatgc taggcgggtg cccgaaggag
      361 cgagctgaga tctctatgtt ggaaggggcc gtgctggata tccgctacgg cgtgagccgc
      421 attgcatact caaaggattt cgagaccctc aaggttgact tcctgtcaaa gctgcctgaa
      481 atgctaaaga tgttcgaaga ccgtctctgt cataagacct acctgaacgg ggaccacgtg
      541 acacacccgg actttatgtt gtatgacgct ctggacgtcg tgctttatat ggaccctatg
      601 tgcctggacg cttttcccaa actagtgtgt ttcaagaaac gtatcgaagc tatccctcag
      661 attgataagt acctgaagtc ctcaaagtac atcgcctggc cccttcaagg t
//
