LOCUS       sequence-53167-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_L1914N_V2048T
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             124..777
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             850..870
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccacg agaaccttta tttccaagga
      121 tcaatggcac cgatcctggg gtattggaag atcaagggcc tagtgcagcc cactcgtctg
      181 ctcctggagt acctagaaga gaagtatgag gagcaccttt atgagcggga cgagggtgac
      241 aagtggcgca ataagaaatt cgagctaggc ttggaattcc ccaatctgcc ttattacatc
      301 gatggcgacg ttaagttgac tcagtccatg gccatcatcc gctacatcgc cgacaagcac
      361 aatatgctag gcgggtgccc gaaggagcga gctgagatct ctatgttgga aggggccgtg
      421 ctggatatcc gctacggcgt gagccgcatt gcatactcaa aggatttcga gaccctcaag
      481 gttgacttcc tgtcaaagct gcctgaaatg ctaaagatgt tcgaagaccg tctctgtcat
      541 aagacctacc tgaacgggga ccacgtgaca cacccggact ttatgttgta tgacgctctg
      601 gacgtcgtgc tttatatgga ccctatgtgc ctggacgctt ttcccaaact agtgtgtttc
      661 aagaaacgta tcgaagctat ccctcagatt gataagtacc tgaagtcctc aaagtacatc
      721 gcctggcccc ttcaaggttg gcaagccaca tttggcggtg gcgaccatcc tcccaagtca
      781 gacctagtgc ctaggcacaa ccagaccagc ctatataaga aagccgggtc agctgccgct
      841 gtgctggagg agaacctgta tttccagggc ggatccaaca tcatgctcaa caacgatga
//
