LOCUS       sequence-53171-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_L1914N_L1936Y_V2048T_F2083Y_I2...
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..101
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             102..122
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             126..779
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             852..872
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catctcgcaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
       61 taaatatagg atctatgcac caccatcacc accatcacca cgagaacctt tatttccaag
      121 gatcaatggc accgatcctg gggtattgga agatcaaggg cctagtgcag cccactcgtc
      181 tgctcctgga gtacctagaa gagaagtatg aggagcacct ttatgagcgg gacgagggtg
      241 acaagtggcg caataagaaa ttcgagctag gcttggaatt ccccaatctg ccttattaca
      301 tcgatggcga cgttaagttg actcagtcca tggccatcat ccgctacatc gccgacaagc
      361 acaatatgct aggcgggtgc ccgaaggagc gagctgagat ctctatgttg gaaggggccg
      421 tgctggatat ccgctacggc gtgagccgca ttgcatactc aaaggatttc gagaccctca
      481 aggttgactt cctgtcaaag ctgcctgaaa tgctaaagat gttcgaagac cgtctctgtc
      541 ataagaccta cctgaacggg gaccacgtga cacacccgga ctttatgttg tatgacgctc
      601 tggacgtcgt gctttatatg gaccctatgt gcctggacgc ttttcccaaa ctagtgtgtt
      661 tcaagaaacg tatcgaagct atccctcaga ttgataagta cctgaagtcc tcaaagtaca
      721 tcgcctggcc ccttcaaggt tggcaagcca catttggcgg tggcgaccat cctcccaagt
      781 cagacctagt gcctaggcac aaccagacca gcctatataa gaaagccggg tcagctgccg
      841 ctgtgctgga ggagaacctg tatttccagg gcggatccaa catcatgct
//
