LOCUS       sequence-53173-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB017
            NP_9409803_LRRK2_1867_2161_L1914N_V204...
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..103
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             104..124
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             128..781
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             854..874
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 accatctcgc aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
       61 tataaatata ggatctatgc accaccatca ccaccatcac cacgagaacc tttatttcca
      121 aggatcaatg gcaccgatcc tggggtattg gaagatcaag ggcctagtgc agcccactcg
      181 tctgctcctg gagtacctag aagagaagta tgaggagcac ctttatgagc gggacgaggg
      241 tgacaagtgg cgcaataaga aattcgagct aggcttggaa ttccccaatc tgccttatta
      301 catcgatggc gacgttaagt tgactcagtc catggccatc atccgctaca tcgccgacaa
      361 gcacaatatg ctaggcgggt gcccgaagga gcgagctgag atctctatgt tggaaggggc
      421 cgtgctggat atccgctacg gcgtgagccg cattgcatac tcaaaggatt tcgagaccct
      481 caaggttgac ttcctgtcaa agctgcctga aatgctaaag atgttcgaag accgtctctg
      541 tcataagacc tacctgaacg gggaccacgt gacacacccg gactttatgt tgtatgacgc
      601 tctggacgtc gtgctttata tggaccctat gtgcctggac gcttttccca aactagtgtg
      661 tttcaagaaa cgtatcgaag ctatccctca gattgataag tacctgaagt cctcaaagta
      721 catcgcctgg ccccttcaag gttggcaagc cacatttggc ggtggcgacc atcctcccaa
      781 gtcagaccta gtgcctaggc acaaccagac cagcctatat aagaaagccg ggtcagctgc
      841 cgctgtgctg gaggagaacc tgtatttcca gggcggatcc aacatcatgc tcaacaacga
      901 tgagctcgaa tttgaacaag ctccagaatt
//
