LOCUS       sequence-53176-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            LRRK2_1867_2161_L1914A_L1976Y_C2024S_C2025S_I2049Q_F2083A
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..89
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             114..767
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             840..860
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatataggat
       61 ctatgcacca ccatcaccac catcaccacg agaaccttta tttccaagga tcaatggcac
      121 cgatcctggg gtattggaag atcaagggcc tagtgcagcc cactcgtctg ctcctggagt
      181 acctagaaga gaagtatgag gagcaccttt atgagcggga cgagggtgac aagtggcgca
      241 ataagaaatt cgagctaggc ttggaattcc ccaatctgcc ttattacatc gatggcgacg
      301 ttaagttgac tcagtccatg gccatcatcc gctacatcgc cgacaagcac aatatgctag
      361 gcgggtgccc gaaggagcga gctgagatct ctatgttgga aggggccgtg ctggatatcc
      421 gctacggcgt gagccgcatt gcatactcaa aggatttcga gaccctcaag gttgacttcc
      481 tgtcaaagct gcctgaaatg ctaaagatgt tcgaagaccg tctctgtcat aagacctacc
      541 tgaacgggga ccacgtgaca cacccggact ttatgttgta tgacgctctg gacgtcgtgc
      601 tttatatgga ccctatgtgc ctggacgctt ttcccaaact agtgtgtttc aagaaacgta
      661 tcgaagctat ccctcagatt gataagtacc tgaagtcctc aaagtacatc gcctggcccc
      721 ttcaaggttg gcaagccaca tttggcggtg gcgaccatcc tcccaagtca gacctagtgc
      781 ctaggcacaa ccagaccagc ctatataaga aagccgggtc agctgccgct gtgctggagg
      841 agaacctgta tttccagggc ggatccaa
//
