LOCUS       sequence-53185-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG412
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..99)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaacaaaata tggaaaatac ggcaaaatta ggatccacta gtacctccac ctccgctgtc
       61 catgtgctgg cgttcgaatt tagcagcagc ggtttctttg aatttggcgg cgccctgaaa
      121 ataaagattc tctctcgagc ggccgccacc ggtggagctc caccgatccc ccgggcacca
      181 tgcttgactt ggtagaggac tgtatcttgc aactgcgtgt tcgtatagct taactttgtt
      241 gtaatccgaa tcgtttccta gtatttcaat tctgtgatca acaaagtgtg catctggaag
      301 ttggacgacc ttctttgctt tgtaagtagt tttaaaatcg caaaggtagt gacctccacc
      361 ttctagtagt agtgccatgt taatgttctg aaaatttaaa tataatccgt tagttagtaa
      421 gtccaagttt aaacttactc caacaagtag tccatctcta acatgtagtt tttcagtcga
      481 tggttcccac ttaagagtct tcttttgcat aactggtccg ttaggaggaa agttagttcc
      541 tttaaatctg acattttgga agaagcaatc accttctaga ctaatatcac ttctctgaaa
      601 atttaaatat gtatggttag ttagtaccga actgtttaaa cttacaatag tgcaaatacc
      661 tttatcttcg aaagtcatag ttctttccca cgagtatcct tctggaaacg attgct
//
