LOCUS       sequence-53191-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Guan B, Wang TL, Shih IeM
  TITLE     ARID1A, a factor that promotes formation of SWI/SNF-mediated 
            chromatin remodeling, is a tumor suppressor in gynecologic cancers.
  JOURNAL   Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.
  PUBMED    21900401
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2011-11-1"; volume: "71(21)"; pages: "6718-27. Epub 
            2011 Sep 7"
COMMENT     Sequence Label: pLenti-puro-LacZ
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(457..481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             534..575
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(625..642)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(654..674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc
       61 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
      121 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
      181 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
      241 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg
      301 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggagacgact
      361 cctggagccc gtcagtatcg gcggaattac agctgagcgc cggtcgctac cattaccagt
      421 tggtctggtg tcaaaaaact aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
      481 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc
      541 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
      601 attgagttta aacctgctga tcagcctcga ctgtgccttc tagtcgaccc tgtggaatgt
      661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      901 t
//
