LOCUS       sequence-53199-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Huang DC, Holtz WJ, Maharbiz MM
  TITLE     A genetic bistable switch utilizing nonlinear protein degradation.
  JOURNAL   J Biol Eng. 2012 Jul 9;6(1):9.
  PUBMED    22776405
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2012-07-9"; volume: "6(1)"; pages: "9"
COMMENT     Sequence Label: PRM-GFP
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             25..36
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        174..278
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(497..516)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcttctcct ttgctcatgg tacctttctc ctctttaatg aattgagatc cacgttaaat
       61 ctatcaccac tatttataaa tatctaacac cgtgcgtgtt gactatttta cctctggcgg
      121 tgataatggt tgcagatctc atgaattcag gtggcacttt tcggggaaat gtgcgcggaa
      181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      301 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      361 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      421 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      481 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      541 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      601 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      661 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
      721 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
//
