LOCUS       sequence-53200-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Huang DC, Holtz WJ, Maharbiz MM
  TITLE     A genetic bistable switch utilizing nonlinear protein degradation.
  JOURNAL   J Biol Eng. 2012 Jul 9;6(1):9.
  PUBMED    22776405
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2012-07-9"; volume: "6(1)"; pages: "9"
COMMENT     Sequence Label: PRM-GFP
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(157..184)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(200..271)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(310..342)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     primer_bind     complement(383..403)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct
       61 ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca
      121 gtgagcgagg aagcggaata tatccctagg tataaacgca gaaaggccca cccgaaggtg
      181 agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      241 agtctttcga ctgagccttt cgttttattt gatgcctgga gatccttact cgagtcagga
      301 tccttattaa gctactaaag cgtagttttc gtcgtttgca gctttgtaga gctcatccat
      361 gccatgtgta atcccagcag cagttacaaa ctcaagaagg accatgtggt cacgcttttc
      421 gttgggatct ttcgaaagga cagattgtgt cgacaggtaa tggttgtctg gtaaaaggac
      481 agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa cggaaccatc
      541 ttcaacgttg tggcgaattt tgaagttagc tttgattcca ttcttttgtt tgtctgccgt
      601 gatgtataca ttgtgtgagt taaagttgta ctcaagtttg tgtccaagaa tgtttccatc
      661 ttctttaaaa tcaataccct ttaactcgat acgattaaca agggtatcac cttcaaactt
      721 gacttcagca cgcgtcttgt aggtcccgtc atctttgaaa gatatagtgc gttcctgtac
      781 ataaccttcg ggcatggcac tcttgaaaaa gtcatgccgt ttcatgtgat ccggataacg
      841 ggaaaagcat tgaacaccat aggtcagagt agtgacaagt gttggccacg gaacaggtag
      901 tttt
//
