LOCUS       sequence-53202-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Huang DC, Holtz WJ, Maharbiz MM
  TITLE     A genetic bistable switch utilizing nonlinear protein degradation.
  JOURNAL   J Biol Eng. 2012 Jul 9;6(1):9.
  PUBMED    22776405
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2012-07-9"; volume: "6(1)"; pages: "9"
COMMENT     Sequence Label: PLlacO-1-CImf
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..47)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(167..877)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggagatc
       61 cttactcgag tcaggatcct tattacgcaa acgcataatt cgcctggccc gcattcagca
      121 taaaggtcgg cacttcattg gtattttctt catttttatt cgccgcgcca aacgtctctt
      181 caggccactg actagcgata actttcccca caacggaaca actctcattg catgggatca
      241 ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct
      301 tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct
      361 gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg
      421 cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg
      481 tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt gagaacatcc
      541 ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac
      601 taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc
      661 taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca ttgatgccat
      721 taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg
      781 ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa
      841 gctgctcttg tgttaatggt ttcttttttg tgctcatgag gccgtcgcga gag
//
