LOCUS       sequence-53205-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Huang DC, Holtz WJ, Maharbiz MM
  TITLE     A genetic bistable switch utilizing nonlinear protein degradation.
  JOURNAL   J Biol Eng. 2012 Jul 9;6(1):9.
  PUBMED    22776405
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2012-07-9"; volume: "6(1)"; pages: "9"
COMMENT     Sequence Label: PLlacO-1-CImf
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    59..75
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             138..848
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagaattgtg agcggataac aattgacatt
       61 gtgagcggat aacaagatac tgagcacgaa ttcaaaagat ctaatccaac aatatagcat
      121 actctcgcga cggcctcatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg
      181 acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg
      241 aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca
      301 tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg
      361 aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc
      421 agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt
      481 tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca
      541 aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa
      601 caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg
      661 ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac
      721 tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc
      781 catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga
      841 cgtttggcgc ggcgaataaa aatgaa
//
