LOCUS       sequence-53216-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Huang DC, Holtz WJ, Maharbiz MM
  TITLE     A genetic bistable switch utilizing nonlinear protein degradation.
  JOURNAL   J Biol Eng. 2012 Jul 9;6(1):9.
  PUBMED    22776405
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2012-07-9"; volume: "6(1)"; pages: "9"
COMMENT     Sequence Label: SW5
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..52)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
ORIGIN
        1 attcatgaga tctttattaa gctactaaag cgtagttttc gtcgtttgca gcttttgttt
       61 taaaaactat tgcaaatacc tcttcatatt tttcagcagg tataattttt agttttgctt
      121 ttacttcatc tggaatttcg tctaagtctc tttcattttt ctttggaata atgattgttt
      181 tcaatccact tcttgaagca gaaattgatt tttctcttaa tccaccaatt ggcaatacat
      241 ttcctcttag tgtgatttca ccagtcattc caatttcttt tgaaactggt ttatctgaaa
      301 gtgcagaaat taaagcagtt gtaattgtga taccagcact tggtccatct tttggaacag
      361 ctccttcagg aacgtgaaca tgaatatcat tttcttcaaa tactttttta tctactccaa
      421 atttttcaaa gtttgattta acataagtta acgcaattgt tgcagattct ttcataactt
      481 caccaagttt acctgtaaga attaaatttc ctttaccagg gtataaactt acttcaattg
      541 gtaaaatatc tccaccaaat tgtgtatatg ctaaacctgt aaccacacca atttgagatt
      601 cttcttgttt ttcagtgtga tcaaaaattc ttttacctaa taaatcattt acttgttttt
      661 catcaataac aattttatcc atttcaccat ttagattttt aacaatatac tttctaaat
//
