LOCUS       sequence-53217-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_L1914A
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..90
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             115..768
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             841..861
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatatagga
       61 tctatgcacc accatcacca ccatcaccac gagaaccttt atttccaagg atcaatggca
      121 ccgatcctgg ggtattggaa gatcaagggc ctagtgcagc ccactcgtct gctcctggag
      181 tacctagaag agaagtatga ggagcacctt tatgagcggg acgagggtga caagtggcgc
      241 aataagaaat tcgagctagg cttggaattc cccaatctgc cttattacat cgatggcgac
      301 gttaagttga ctcagtccat ggccatcatc cgctacatcg ccgacaagca caatatgcta
      361 ggcgggtgcc cgaaggagcg agctgagatc tctatgttgg aaggggccgt gctggatatc
      421 cgctacggcg tgagccgcat tgcatactca aaggatttcg agaccctcaa ggttgacttc
      481 ctgtcaaagc tgcctgaaat gctaaagatg ttcgaagacc gtctctgtca taagacctac
      541 ctgaacgggg accacgtgac acacccggac tttatgttgt atgacgctct ggacgtcgtg
      601 ctttatatgg accctatgtg cctggacgct tttcccaaac tagtgtgttt caagaaacgt
      661 atcgaagcta tccctcagat tgataagtac ctgaagtcct caaagtacat cgcctggccc
      721 cttcaaggtt ggcaagccac atttggcggt ggcgaccatc ctcccaagtc agacctagtg
      781 cctaggcaca accagaccag cctatataag aaagccgggt cagctgccgc tgtgctggag
      841 gagaacctgt atttccaggg cggatccaac
//
