LOCUS       sequence-53218-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-tdTomato
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..599
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(64..84)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(482..493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    642..763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(653..672)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     707..726
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
       61 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
      121 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
      181 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
      241 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
      301 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
      361 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
      421 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
      481 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
      541 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
      601 tcgataccgt cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
      661 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      721 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      781 tcattttatg tttcaggttc agggggagat gtgggag
//
