LOCUS       sequence-53221-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_L1916N_L1936R_L1976A_I2049A_L2...
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             99..119
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             123..776
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             849..869
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctcgcaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
       61 atataggatc tatgcaccac catcaccacc atcaccacga gaacctttat ttccaaggat
      121 caatggcacc gatcctgggg tattggaaga tcaagggcct agtgcagccc actcgtctgc
      181 tcctggagta cctagaagag aagtatgagg agcaccttta tgagcgggac gagggtgaca
      241 agtggcgcaa taagaaattc gagctaggct tggaattccc caatctgcct tattacatcg
      301 atggcgacgt taagttgact cagtccatgg ccatcatccg ctacatcgcc gacaagcaca
      361 atatgctagg cgggtgcccg aaggagcgag ctgagatctc tatgttggaa ggggccgtgc
      421 tggatatccg ctacggcgtg agccgcattg catactcaaa ggatttcgag accctcaagg
      481 ttgacttcct gtcaaagctg cctgaaatgc taaagatgtt cgaagaccgt ctctgtcata
      541 agacctacct gaacggggac cacgtgacac acccggactt tatgttgtat gacgctctgg
      601 acgtcgtgct ttatatggac cctatgtgcc tggacgcttt tcccaaacta gtgtgtttca
      661 agaaacgtat cgaagctatc cctcagattg ataagtacct gaagtcctca aagtacatcg
      721 cctggcccct tcaaggttgg caagccacat ttggcggtgg cgaccatcct cccaagtcag
      781 acctagtgcc taggcacaac cagaccagcc tatataagaa agccgggtca gctgccgctg
      841 tgctggagga gaacctgtat ttccagggcg
//
