LOCUS       sequence-53224-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  TITLE     mouse ZO-1 shRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mZO1-2 shRNA
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    264..297
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(339..355)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(340..356)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(412..724)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(473..493)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    779..896
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tctgatggtt ctctagaaac tgctgagggc gggaccgcat ctggggacca tctgttcttg
       61 gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca tattctgctg
      121 tctctctgtt cctaaccttg atctgaactt ctctattctc agttatgtat tttccatgcc
      181 ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc tttcagcggc
      241 cgcttaagct tggaaccctt aatataactt cgtataatgt atgctatacg aagttattag
      301 gtccctcgac ctgctggaat ctcgtgaagc gagcttatcg ataccgtcga cctcgagaaa
      361 aaagaacaga gctgaacagt tatctcttga acaactgttc agctctgttc aaacaaggct
      421 tttctccaag ggatatttat agtctcaaaa cacacaatta ctttacagtt agggtgagtt
      481 tccttttgtg ctgtttttta aaataataat ttagtatttg tatctcttat agaaatccaa
      541 gcctatcatg taaaatgtag ctagtattaa aaagaacaga ttatctgtct tttatcgcac
      601 attaagcctc tatagttact aggaaatatt atatgcaaat taaccggggc aggggagtag
      661 ccgagcttct cccacaagtc tgtgcgaggg ggccggcgcg ggcctagaga tggcgcgtcg
      721 gatctctaga gcgcggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa
      781 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
      841 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt
      901 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat
      961 cgaat
//
