LOCUS       sequence-53225-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_L1916N_L1976A_I2049A_V2074A_L2...
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..88
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa atataggatc
       61 tatgcaccac catcaccacc atcaccacga gaacctttat ttccaaggat caatggcacc
      121 gatcctgggg tattggaaga tcaagggcct agtgcagccc actcgtctgc tcctggagta
      181 cctagaagag aagtatgagg agcaccttta tgagcgggac gagggtgaca agtggcgcaa
      241 taagaaattc gagctaggct tggaattccc caatctgcct tattacatcg atggcgacgt
      301 taagttgact cagtccatgg ccatcatccg ctacatcgcc gacaagcaca atatgctagg
      361 cgggtgcccg aaggagcgag ctgagatctc tatgttggaa ggggccgtgc tggatatccg
      421 ctacggcgtg agccgcattg catactcaaa ggatttcgag accctcaagg ttgacttcct
      481 gtcaaagctg cctgaaatgc taaagatgtt cgaagaccgt ctctgtcata agacctacct
      541 gaacggggac cacgtgacac acccggactt tatgttgtat gacgct
//
