LOCUS       sequence-53230-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ATAD2B
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             571..588
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(641..659)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      655..702
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(826..845)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggaagatc aggaagaaaa
      121 taccctgcgc gaactgcgcc tgtttctgcg tgatgtgacc aaacgtctgg cgaccgataa
      181 acgttttaat atttttagca aaccggtgga tattgaagaa gtgagcgatt atctggaagt
      241 gattaaagaa ccgatggatc tgagcaccgt gattaccaaa atcgataaac ataattatct
      301 gaccgcgaaa gatttcctga aagatattga tctgatttgc agcaacgcgc tggaatataa
      361 cccggataaa gatccgggtg ataaaattat tcgtcatcgc gcgtgtaccc tgaaagatac
      421 cgcgcatgcg attatcgccg ccgaactgga tccggaattt aacaaactgt gcgaagaaat
      481 caaagaagcg cgtattaaac gtggctgaca gtaaaggtgg atacggatcc gaattcgagc
      541 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      601 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      661 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      721 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      781 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      841 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      901 ctttagggtt cgatt
//
