LOCUS       sequence-53231-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SRPK2
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..103
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc atcatcattc
       61 ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgggta gcgatgacga
      121 ggagcaggaa gatcccgccg attactgtaa aggcggttat catccagtga agattggcga
      181 cttgttcaac ggtcgttacc acgttatccg caaactgggc tggggtcact tttccactgt
      241 ctggttgtgc tgggacatgc aaggcaagcg tttcgtagct atgaaagtgg taaaatctgc
      301 gcagcactat acagaaaccg cactggatga aattaagttg ctgaaatgtg tccgcgagtc
      361 agatccctcg gacccgaata aggacatggt agtgcaactg atcgatgact ttaagatttc
      421 gggtatgaac ggcatccatg tttgcatggt attcgaagtg ttgggtcacc atctgttgaa
      481 atggattatc aagagcaatt accagggcct gcccgtacgt tgcgtgaaat ccattatccg
      541 ccaagttttg cagggcctgg attatctgca ctctaagtgc aaaatcattc atactgacat
      601 caaaccagag aacattttga tgtgcgtcga tgacgcctac gtacgtcgca tggctgcgga
      661 agcaacagag tggcagaagg ccggcgctcc tccgccctca ggtagtgcgg tgtcgaccgc
      721 cccagcagct gatctgttgg ttaatcctct ggacccgcgt aacgccgaca aaatccgcgt
      781 caagattgcg gacctgggca acgcatgctg ggtgcacaaa cactttactg aagatatcca
      841 aa
//
