LOCUS       sequence-53232-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SEPX1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             479..496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(549..567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      563..610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(734..753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgaaggtt ttccagaatc
      121 actttgaacc tggcgtttac gtgtgtgcca agtgtggcta tgagctgttc tccagccgct
      181 cgaagtatgc acactcgtct ccatggccgg cgttcaccga gaccattcac gccgacagcg
      241 tggccaagcg tccggagcac aatagatctg aagccttgaa ggtgtcctgt ggcaagtgtg
      301 gcaatgggtt gggccacgag ttcctgaacg acggccccaa gccggggcag tcccgattct
      361 caatattcag cagctcgctg aagtttgtcc ctaaaggcaa agacacttct gcctgacagt
      421 aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      481 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      601 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      781 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      841 ctcgacccca aaaaacttga ttagggtgat ggttcacgtt agtgggccat cgccctgata
//
