LOCUS       sequence-53233-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHIP
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             568..585
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(638..656)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      652..699
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(823..842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggatcatc agccgcgtcg
      121 tcgtctgcgc aaccgtgcgc agagctatga tatccaggcg tggaaaaaac agtgcgaaga
      181 actgctgaat ctgatttttc agtgtgaaga tagcgaaccg tttcgtcagc cggttgatct
      241 gctggaatat ccggattatc gcgatatcat tgataccccg atggattttg cgaccgtgcg
      301 cgaaaccctg gaagcgggta actatgaaag cccgatggaa ctgtgcaaag atgtgcgcct
      361 gatttttagc aacagcaaag cctatacccc gagcaaacgt agccgtatct atagcatgag
      421 cctgcgtctg agcgcgtttt ttgaagaaca cattagcagc gtgctgagcg attataaaag
      481 cgcgctgcgc tttcataaac gttgacagta aaggtggata cggatccgaa ttcgagctcc
      541 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      601 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      661 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      721 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      781 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      841 cttcctttct cgcccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      901 ttaggg
//
