LOCUS       sequence-53237-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: H2AFY2
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..404
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             411..431
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtatg agcgataaaa ttattcacct gactgacgac agttttgaca
      121 cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag tggtgcggtc
      181 cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag ggcaaactga
      241 ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat ggcatccgtg
      301 gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa gtgggcgcac
      361 tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccggtacc gagaacttgt
      421 acttccaatc catgaaagaa ggaacttcaa attccacctc tgaagatggg ccaggggatg
      481 gattcaccat tctgtcttct aagagccttg ttctgggaca gaagctgtcc ttaacccaga
      541 gtgacatcag ccatattggc tccatgagag tggagggcat tgtccaccca accacagccg
      601 aaattgacct caaagaagat ataggtaaag ccttggaaaa ggctggggga aaagagttct
      661 tggaaacggt aaaggagctt cgcaaatccc aaggcccttt ggaagtcgcc gaagccgccg
      721 tcagccaatc cagtggactc gcagccaaat ttgtcatcca ctgtcacatc cctcagtggg
      781 gctccgacaa atgtgaagaa cagcttgaag agaccatcaa aaactgcctg tcagcggcgg
      841 aggacaagaa gctaaagtcc gtcgcgttcc cgcctttccc cagcggcaga aactgctttc
      901 ccaaacagac tgcggcccag gtgaccctca aagccatctc agcccacttt gatgactcga
      961 gcgcgtcctc gctgaagaac gtgtacttcc tgctcttcga cagcgagagc atcggcatct
     1021 acgtgcagga gatggcca
//
